ISOLATION AND CHARACTERIZATION OF CATECHIN AND CATECHIN-3-O-GALLATE FROM THE BARK OF Vitellaria paradoxa (SAPOTACEAE)

Authors

  • S. Y. Maiyama Department of Energy and Applied Chemistry, Faculty of Chemical and Life Sciences, Usmanu Danfodiyo University Sokoto, P.M.B. 2346, Sokoto State, Nigeria.
  • L. G. Hassan Department of Pure and Environmental Chemistry, Faculty of Chemical and Life Sciences, Usmanu Danfodiyo University Sokoto, P.M.B. 2346, Sokoto State, Nigeria.
  • I. Atiku Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, Ahmadu Bello University Zaria, P.M.B 1013, Kaduna State, Nigeria.
  • K. J. Umar Department of Pure and Industrial Chemistry, Faculty of Sciences, Federal University Birnin Kebbi, P.M.B. 1157, Kebbi State, Nigeria.
  • A. Babagana Department of Chemistry, Faculty of Sciences, Borno State University, P.M.B. 1122, Njimtilo, Maiduguri, Nigeria.

Keywords:

Vitellaria paradoxa, catechin, catechin-3-O-gallate, LC-MS, Spectroscopy, NMR, HSQC, COSY

Abstract

Background: The emergence of drug-resistant microorganisms has led to the screening of medicinal plants for alternative. Vitellaria paradoxa of Sapotaceae family, is a medicinal plant of vast use in traditional medicine for the treatment of many diseases including cancer, bacterial and fungal infections. The aim of this study is to isolate and characterize bioactive compounds from the ethyl acetate stem bark extract of V. paradoxa.

Methods: The powdered stem bark was extracted successively by maceration using n-hexane, ethyl acetate and ethanol. The dichloromethane soluble portion of the ethyl acetate extract (MD) was subjected to column chromatography and fraction (coded MD168) obtained by pooling of fractions based on their TLC profiles was purified and characterized using Liquid Chromatography-Mass Spectrometer (LC-MS), one dimensional (1D) and two dimensional (2D) Nuclear Magnetic Resonance (NMR) techniques including Heteronuclear Single Quantum Coherence (HSQC), Heteronuclear Multiple Bond Correlation (HMBC) and Proton-Proton Correlation Spectroscopy (1H-1H COSY) and by comparison with the literature.

Results: Based on the spectral data obtained, MD168 was identified as a mixture of two known types of flavanol of flavonoid class: catechin and catechin-3-O-gallate.

Conclusion: The two compounds were previously isolated from the other parts and extracts of V. paradoxa and were reported to be active against strains which make them potential precursors for new drug synthesis.

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Published

2024-12-21

Issue

Vol. 1 No. 2 (2024): Cavendish University Journal of Science and Technology

Section

Articles